Fig 1: Upregulation of NCL promotes SVV replication. (A) Fluorescence of packed lentiviruses in HEK-293FT cells, and lentivirus-transduced BHK-21 cells expressing GFP, NCL-GFP, and FBL-GFP were captured with fluorescence microscopy at 24 h posttransfection and transduction. (B) Cell viability of lentivirus transduced BHK-21 cells were determined by CCK-8 assay (NS, not significant). (C to D) BHK-21 cells were transduced with lentivirus expressing GFP, NCL-GFP, and FBL-GFP. Cells were infected with SVV (MOI = 5) after 48 h postransduction, and the expression of VP1 was analyzed by TCID50 assay and Western blotting with antibodies to GFP, VP1, and ß-actin at 0, 6, and 12 hpi, respectively. Statistical analysis was performed using GraphPad Prism. The error bars indicated mean ± SD from three independent experiments (*, P < 0.05; ***, P < 0.001; NS, not significant). (E) The expression of VP1 was quantified normalized against ß-actin using Image J. Statistical analysis was performed using GraphPad Prism. The error bars indicated mean ± SD from three independent experiments (*, P < 0.05; ***, P < 0.001; NS, not significant).